Detection of HTLV type I provirus by in situ polymerase chain reaction in mouthwash mononuclear cells of HAM/TSP patients and HTLV type I carriers.
Journal - AIDS research and human retroviruses (UNITED STATES )
Molecular studies have revealed the presence of HTLV-I provirus DNA in saliva of HTLV-I-infected subjects. However, cellular localization has not been determined. In the present study, we have used in situ PCR technique to study saliva-associated cells for localization of HTLV-I proviral DNA. We found that HTLV-I proviral DNA was present in the nuclei and cytoplasm of salivary lymphocytes in five (71%) of seven HTLV-I-seropositive subjects. The percentage of infected cells in positive mouthwash samples ranged from 0.5 to 2%. None of the HTLV-I-negative patients had HTLV-I provirus in saliva. The localization of HTLV-I provirus DNA suggests that salivary lymphocytes can serve as vector for HTLV-I infection through saliva.
|ISSN : ||0889-2229|
|Mesh Heading : ||Carrier State DNA, Viral HTLV-I Infections Human T-lymphotropic virus 1 Humans Lymphocytes Mouthwashes Paraparesis, Tropical Spastic Polymerase Chain Reaction Proviruses Saliva isolation & purification genetics genetics isolation & purification cytology|
|Mesh Heading Relevant : ||virology isolation & purification virology virology methods virology|