Screening Method for Detection of Drugsof Abuse in Human Urine
(1971)
Journal - Clinical Chemistry
Abstract :
A simple, convenient method is described for the detectionin human urine
of three major classes of drugs of abuse: amphetamines,barbiturates, and
alkaloids. The drugs are adsorbed from urineby a column of non-ionic
resin (Amberlite XAD-2), eluted withmethanol, and chromatographed
on a thin-layer silica gel plate.The drugs are made visible and identified
by a series of proceduresinvolving exposure to ultraviolet radiation, heat
treatment,sequential spraying with group-specific reagents, and measurementof Rf values. Detection limits for 5-ml urine specimens are:0.4 µg
per ml for amphetamines and barbiturates, and 0.8g per ml for alkaloids.
The test requires 6 h to perform. Onetechnologist can process 50 specimens
per day.
| Keywords : | non-ionic resin chromatography • thin-layer chromatography on silica gel • amphetamines, barbiturates, alkaloids • Rf values, TLC |
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FOLLOW-UP & TOXICITY BLOOD DETERMINATIONS--LRC PATIENTS
(1981)
| Project Number : | 3N01HV032961-01273 |
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| ICD : | DIVISION OF HEART AND VASCULAR DISEASES |
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FOLLOW-UP & TOXICITY BLOOD DETERMINATIONS--LRC PATIENTS
(1980)
| Project Number : | 2N01HV032961-01173 |
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| ICD : | DIVISION OF HEART AND VASCULAR DISEASES |
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