Immune responses of mice to influenza subunit vaccine in combination with CIA07 as an adjuvant.
Journal - Microbiology and immunology (Japan )
CIA07 is an immunostimulatory agent composed of E. coli DNA fragments and modified LPS lacking the lipid A moiety. In this study, we investigated whether CIA07 promotes immune responses as an adjuvant to the influenza subunit vaccine. Balb/c mice were immunized intramuscularly once or twice at a 4-week interval with the trivalent influenza subunit vaccine antigen alone or in combination with CIA07 as adjuvant. Antigen-specific serum antibody titers and hemagglutination-inhibition (HI) antibody titers were assessed. At 4 weeks after each immunization, the antigen-specific total serum IgG antibody titer in mice receiving CIA07 was 2 to 3 times higher than that in animals administered antigen alone (P<0.05). The CIA07-treated group additionally displayed higher HI antibody titers against each of the 3 vaccine strains, compared to the antigen group. Animals receiving antigen alone displayed barely detectable antigen-specific serum IgG2a antibody titers. In contrast, coadministration of CIA07 with antigen led to significantly enhanced IgG2a antibody responses, suggesting that CIA07 stimulates a Th1-type immune response. Moreover, the CIA07-treated group displayed a marked increase in the number of interferon gamma-producing CD8(+) T cells in splenocytes. These data collectively demonstrate that CIA07 has the ability to induce both Th1-type cellular and Th2-type humoral immune responses to the influenza subunit vaccine, and support its potential as an effective adjuvant to the influenza vaccine.
|ISSN : ||0385-5600|
|Mesh Heading : ||Adjuvants, Immunologic Animals Antibodies, Viral Antibody Formation Antigens, Viral CD8-Positive T-Lymphocytes Hemagglutination Inhibition Tests Immunoglobulin G Influenza A Virus, H1N1 Subtype Influenza Vaccines Lipopolysaccharides Lymphocytes Mice Mice, Inbred BALB C Th1 Cells Vaccination immunology immunology analysis immunology chemistry immunology immunology immunology immunology|
|Mesh Heading Relevant : ||pharmacology analysis pharmacology|
Comparative proteomic analysis of Helicobacter pylori strains associated with iron deficiency anemia.
Journal - Proteomics (Germany )
Helicobacter pylori is known to cause chronic gastritis, peptic ulcer, and gastric cancer, and has also been linked to iron deficiency anemia (IDA). To determine whether H. pylori clinical isolates correlate with the prevalence of H. pylori-associated IDA, we compared the proteomic profiles of H. pylori strains isolated from antral biopsy specimens of H. pylori-positive patients with or without IDA. Fifteen strains, including eight non-IDA and seven IDA strains, were cultured under iron-rich and iron-depleted conditions and then analyzed for protein expression profiles by 2-DE. The distances between two H. pylori strains were determined on the basis of similarities between their expression patterns of 189 protein spots, and a phylogenetic tree was constructed. The results revealed that the IDA strains formed a cluster separate from that of six non-IDA strains, with two non-IDA strains between the clusters. H. pylori strain 26695 was located in the non-IDA cluster. Protein spots displaying similar expression patterns were clustered, and 18 spots predominantly expressed in IDA strains were identified by MALDI-TOF analysis. These data indicate that the non-IDA and IDA strains can be distinguished by their protein expression profiles, suggesting that the polymorphism of H. pylori strains may be one of the factors determining the occurrence of H. pylori-associated IDA.
|ISSN : ||1615-9853|
|Mesh Heading : ||Adolescent Anemia, Iron-Deficiency Bacterial Proteins Biopsy Child Electrophoresis, Gel, Two-Dimensional Helicobacter Infections Helicobacter pylori Humans Phylogeny Proteome Pyloric Antrum Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization isolation & purification metabolism|
|Mesh Heading Relevant : ||microbiology analysis microbiology chemistry analysis|