Eucommia ulmoides Oliver leaf extract increases endogenous antioxidant activity in type 2 diabetic mice.
Journal - Journal of medicinal food (United States )
Eucommia ulmoides Oliver (Du-zhong) leaf extract was investigated for its antioxidant effects in type 2 diabetic animals, C57BL/KsJ-db/db mice. Du-zhong extract equivalent to 1% dried whole Du-zhong leaf (0.187 g of extract/100 g of diet) was added to the experimental diets for 6 weeks. The Du-zhong extract supplement significantly lowered blood glucose concentrations and elevated plasma paraoxonase activity compared with the control group. The activities of erythrocyte superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were significantly higher in the Du-zhong group compared with the control group, while glutathione reductase (GR) activity was not different between groups. The activities of SOD, GSH-Px, and GR in liver and kidney were not affected by Du-zhong extract supplementation, whereas the CAT activity was significantly higher in the Du-zhong group than in the control group. Du-zhong extract supplementation resulted in lower levels of hydrogen peroxide and lipid peroxide in erythrocytes, liver, and kidney. These results suggest that the antioxidant activity of Du-zhong extract is potentially beneficial for the prevention and management of complications of type 2 diabetes.
|ISSN : ||1096-620X|
|Mesh Heading : ||Animals Antioxidants Aryldialkylphosphatase Blood Glucose Catalase Diabetes Mellitus, Type 2 Diet Erythrocytes Eucommiaceae Glutathione Peroxidase Glutathione Reductase Hydrogen Peroxide Kidney Lipid Peroxides Liver Male Mice Mice, Inbred C57BL Plant Extracts Plant Leaves Random Allocation Superoxide Dismutase blood analysis blood blood enzymology chemistry enzymology blood metabolism blood metabolism analysis blood chemistry enzymology analysis blood chemistry enzymology blood metabolism|
|Mesh Heading Relevant : ||Phytotherapy metabolism drug therapy chemistry therapeutic use chemistry|
Genistein and daidzein modulate hepatic glucose and lipid regulating enzyme activities in C57BL/KsJ-db/db mice.
Journal - Life sciences (England )
This study examines whether anti-diabetic effects of genistein and daidzein are mediated by hepatic glucose and lipid regulating enzyme activities in type 2 diabetic animals. Male C57BL/KsJ-lepr(db)/lepr(db) (db/db) mice and age-matched non-diabetic littermates (db/+) were used in this study. The db/db mice were divided into control, genistein (0.02%, w/w) and daidzein (0.02%, w/w) groups. The blood glucose and HbA(1c) levels were significantly lower in the genistein and daidzein groups than in the control group, while glucose tolerance only was significantly improved in the genistein-supplemented group. The plasma insulin and C-peptide levels did not differ significantly between groups, yet the glucagon level was lower in the genistein and daidzein groups compared to that in the control db/db or db/+ group. The genistein and daidzein supplements increased the insulin/glucagon ratio in the type 2 diabetic animals. While the hepatic glucokinase activity was significantly lower in the db/db control group, the glucose-6-phosphatase and phosphoenolpyruvate carboxykinase activities were significantly higher in the control group compared to the db/+ group. Interestingly, these hepatic glucose metabolizing enzyme activities were reversed by the genistein and daidzein supplementation in db/db mice compared to the control group. The hepatic fatty acid synthase, beta-oxidation and carnitine palmitoyltransferase activities were all significantly lower in the genistein and daidzein groups than in the control group. The genistein and daidzein supplements also improved the plasma total cholesterol, triglyceride, HDL-cholesterol/total cholesterol, free fatty acid and hepatic triglyceride concentrations in the db/db mice. These results suggest that genistein and daidzein exert anti-diabetic effect in type 2 diabetic conditions by enhancing the glucose and lipid metabolism.
|ISSN : ||0024-3205|
|Mesh Heading : ||Animals Body Weight C-Peptide Diabetes Mellitus, Type 2 Diet Eating Genistein Glucagon Glucose Glucose Tolerance Test Hemoglobin A, Glycosylated Insulin Isoflavones Leptin Lipid Metabolism Liver Liver Glycogen Mice Mice, Inbred C57BL Phytoestrogens Receptors, Cell Surface Receptors, Leptin drug effects metabolism genetics metabolism metabolism blood metabolism drug effects enzymology metabolism genetics|
|Mesh Heading Relevant : ||metabolism pharmacology metabolism pharmacology drug effects metabolism pharmacology|
Hypoglycemic and hypolipidemic action of Du-zhong (Eucommia ulmoides Oliver) leaves water extract in C57BL/KsJ-db/db mice.
Journal - Journal of ethnopharmacology (Ireland )
The anti-diabetic efficacy of Du-zhong (Eucommia ulmoides Oliver) leaves water extract (WDZ) was investigated in type 2 diabetic animals. The WDZ was given to C57BL/KsJ-db/db mice as a dietary supplement based on 1% dried whole Du-zhong leaves (0.187 g WDZ/100 g standard diet) for 6 weeks. The WDZ supplementation significantly lowered the blood glucose level and enhanced the glucose disposal in an intraperitoneal glucose tolerance test. The plasma insulin and C-peptide levels were significantly higher in the WDZ group than in the control group, while the glucagon level was lower. The hepatic glucokinase activity was significantly higher in the WDZ group, whereas, the glucose-6-phosphatase and phosphoenolpyruvate carboxykinase activities were significantly lower. The WDZ supplementation also significantly lowered the hepatic fatty acid synthase, HMG-CoA reductase and ACAT activities compared to the control group, while it elevated the lipoprotein lipase activity in the skeletal muscle. The WDZ also altered the plasma and hepatic lipid levels by lowering the cholesterol and triglyceride concentrations, while elevating the plasma HDL-cholesterol level. Therefore, these results suggest that WDZ may partly ameliorate hyperglycemia and hyperlipidemia with type 2 diabetes through increasing glycolysis, suppressing gluconeogenesis and the biosynthesis of fatty acid and cholesterol in the liver.
|ISSN : ||0378-8741|
|Mesh Heading : ||Animals Antilipemic Agents Blood Glucose C-Peptide Diabetes Mellitus, Type 2 Drugs, Chinese Herbal Glucagon Gluconeogenesis Glucose Glucose Tolerance Test Glycolysis Hypoglycemic Agents Insulin Lipid Metabolism Lipids Liver Male Mice Mice, Inbred C57BL Mice, Inbred Strains Pancreas Plant Leaves Time Factors therapeutic use blood blood pathology therapeutic use blood drug effects metabolism drug effects therapeutic use blood drug effects blood drug effects enzymology metabolism drug effects pathology|
|Mesh Heading Relevant : ||Eucommiaceae pharmacology drug effects drug therapy pharmacology pharmacology|