Expression of Gadd45a and p53 proteins in human pancreatic cancer: potential effects on clinical outcomes.
(2007)
Journal - Journal of surgical oncology (United States )
Abstract :
BACKGROUND AND OBJECTIVES: p53 gene has a dual role as a trigger of apoptosis and as an initiator of DNA repair. Gadd45a gene assumes the function to maintain the stability of genome. The present study was designed to assess the significance of Gadd45a and p53 protein expression on pancreatic cancer. METHODS: The expression of p53 and Gadd45a was immunohistochemically studied in 59 patients with pancreatic cancer. RESULTS: The positive expression rate of p53 and Gadd45a was 67.8% and 42.4%, respectively. p53 expression in the patients <65 years was significantly higher than that in the patients >or=65 years (P=0.030). Gadd 45a expression showed no correlation to the patients' age. There was no relation for p53 expression to differentiation and TNM stages. Gadd 45a expression showed significant difference in histological differentiation (P=0.007). The median survival time of p53(+) and Gadd45a(+) group was shorter than that of p53(-) and Gadd45a(-) group (P>0.05), respectively. Meanwhile, the median survival time of p53 (+)Gadd45a(+) group was also shorter than that of p53(-)Gadd45a(-) group (P>0.05). CONCLUSIONS: p53 and Gadd45a proteins are both highly expressed in pancreatic cancer. The overexpression of p53 and Gadd45a may have connection with the malignant biologic behavior of pancreatic cancer..(c) 2007 Wiley-Liss, Inc.
| ISSN : | 0022-4790 |
|---|
| Mesh Heading : | Adult Aged Carcinoma, Pancreatic Ductal Cell Cycle Proteins Female Humans Immunoenzyme Techniques Male Middle Aged Nuclear Proteins Pancreatic Neoplasms Prognosis Tumor Suppressor Protein p53 pathology pathology |
|---|
| Mesh Heading Relevant : | metabolism metabolism metabolism metabolism metabolism |
|---|
Clinicopathological significance of p53 and mdm2 protein expression in human pancreatic cancer.
(2005)
Journal - World journal of gastroenterology : WJG (China )
Abstract :
AIM: To study the clinicopathological significance of p53 and mdm2 protein expression in human pancreatic cancer. METHODS: To investigate the expression of p53 and mdm2 in pancreatic cancer by immunohistochemistry, and the relationships between the p53 and mdm2 protein expression and clinicopathological parameters in pancreatic cancer. RESULTS: The positive expression of p53 protein was found in 40 of 59 patients (67.8%) and that of mdm2 protein in 17 of 59 patients (28.8%). No obvious relationships were found between p53 as well as mdm2 expression and sex, tumor site, TNM staging and histological differentiation. p53 expression was increased in patients younger than 65 years old, while mdm2 had no relationship with age. The survival time of the patients with the positive expression of p53 and mdm2 proteins was obviously shorter than the other groups. CONCLUSION: Both p53 and mdm2 presented relatively high expression in human pancreatic cancer. The overexpression of p53 and mdm2 might reflect the malignant proliferation of pancreatic cancer and their co-expression might be helpful to evaluate the prognosis of the patients with pancreatic cancer.
| ISSN : | 1007-9327 |
|---|
| Mesh Heading : | Adult Aged Female Humans Immunohistochemistry Male Middle Aged Nuclear Proteins Pancreatic Neoplasms Prognosis Proto-Oncogene Proteins Proto-Oncogene Proteins c-mdm2 Tumor Markers, Biological Tumor Suppressor Protein p53 |
|---|
| Mesh Heading Relevant : | metabolism metabolism pathology metabolism metabolism metabolism |
|---|
Clinicopathological significance of Bcl-2 and Bax protein expression in human pancreatic cancer.
(2005)
Journal - World journal of gastroenterology : WJG (China )
Abstract :
AIM: To assess the clinicopathological significance of the expression of the apoptosis-inhibitory Bcl-2 protein (pBcl-2) and the apoptosis-promoting Bax protein (pBax) in human invasive ductal carcinomas (IDCs) of the pancreas. METHODS: Fifty-nine surgical specimens of IDCs of the pancreas were stained immunohistochemically to detect pBcl-2 and pBax expressions whose correlation to tumor classification, staging, and prognosis was analyzed by univariate and multivariate analyses. RESULTS: The expression of pBcl-2 and pBax was detected in 21 of 59 (35.6%) and in 29 of 59 (49.2%) patients with IDCs of the pancreas, respectively. Neither pBcl-2 nor pBax alone was correlated to TNM staging and differentiation degree of IDCs of the pancreas according to univariate analysis. By Mantel-Cox test, the median survival time after surgery for pBcl-2(+) and pBcl-2(-) groups were 14.3 and 7.3 mo, respectively (chi(2) = 9.357, P = 0.002) and that for pBax(+) and pBax(-) groups were 12.9 and 10.2 mo, respectively (chi(2) = 0.285, P>0.05). Contingency coefficient between pBcl-2 and pBax expression was 0.298, indicating that there was correlation between them (chi(2) = 5.74, P<0.05). The median survival time after surgery for pBcl-2(+)pBax(+) and pBcl-2(+)pBax(-) groups were 14.3 and 14.1 mo, respectively, and that for pBcl-2(-)pBax(+) and pBcl-2(-)pBax(-) groups were 5.9 and 9.9 mo, respectively. There was a significant difference between pBcl-2(+)pBax(+) and pBcl-2(-)pBax(+) (chi(2) = 5.06, P<0.05), such was the case for pBcl-2(+)pBax(+) and pBcl-2(-)pBax(-) (chi(2) = 7.18, P<0.01). Cox proportional hazards model for multivariate analysis was applied, indicating that pBcl-2, TNM staging, age and pBax were high risk factors of post-surgical survival time. CONCLUSION: Both pBcl-2 and pBax have high expression in IDCs of the pancreas, indicating that co-expression of pBcl-2 and pBax is a good indicator of favorable prognosis in IDCs of the pancreas.
| ISSN : | 1007-9327 |
|---|
| Mesh Heading : | Aged Apoptosis Carcinoma, Ductal Female Humans Male Middle Aged Neoplasm Invasiveness Pancreatic Neoplasms Prognosis Proto-Oncogene Proteins c-bcl-2 bcl-2-Associated X Protein pathology pathology |
|---|
| Mesh Heading Relevant : | metabolism metabolism metabolism |
|---|
[Expressions of p53 and Gadd45a proteins in human pancreatic cancer and their clinicopathological significance]
(2005)
Journal - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (China )
Abstract :
OBJECTIVE: To study the expressions of p53 and Gadd45a proteins and their clinicopathological significance in human pancreatic cancer. METHODS: The expression of p53 and Gadd45a proteins was detected with immunohistochemistry in a series of 59 pancreatic cancers. Their relationships with the clinicopathological parameters including gender, tumor site, TNM stage, histological differentiation, and the prognosis of pancreatic cancer patients were analyzed. RESULTS: The positive expression rate of p53 protein was 67.8% (40/59) and that of Gadd45a protein was 42.4% (25/59). The positive expression rate of p53 protein is significantly higher in patients < 65 years than in patients > or = 65 years (chi squared = 4.711, P = 0.030). Gadd45a expression was not correlated to the age of the patients. No significant difference was found between the expression of p53 proteins and histological differentiation and TNM stage of the tumors. Gadd45a expression was correlated with histological differentiation of pancreatic cancer (chi squared = 10.052, P = 0.007), but not with TNM stage of the tumors. No significant differences in the prognosis were found between the groups with and without p53 expression (chi squared = 0.09, P = 0.764) and the groups with and without Gadd45a expression (chi squared = 0.14, P = 0.704). CONCLUSIONS: Both p53 and Gadd45a are highly expressed in human pancreatic cancer and may be associated with biological features of pancreatic cancer. Their expression alone or co-expression may be not helpful to evaluate the prognosis of patients with pancreatic cancer.
| ISSN : | 1000-503X |
|---|
| Mesh Heading : | Adult Aged Carcinoma, Pancreatic Ductal Cell Cycle Proteins Female Humans Immunohistochemistry Male Middle Aged Neoplasm Staging Nuclear Proteins Pancreatic Neoplasms Tumor Markers, Biological Tumor Suppressor Protein p53 pathology pathology biosynthesis |
|---|
| Mesh Heading Relevant : | metabolism biosynthesis biosynthesis metabolism biosynthesis |
|---|