Identification and functional reconstitution of effector proteins for the GTPases Rac and CDC42Hs.
(1998)
Journal - Methods in molecular biology (Clifton, N.J.) (UNITED STATES )
| ISSN : | 1064-3745 |
|---|
| Mesh Heading : | Amino Acid Sequence Animals Cell Cycle Proteins Consensus Sequence GTP Phosphohydrolases GTP-Binding Proteins Guanosine Triphosphate Humans Molecular Biology Molecular Sequence Data Protein-Serine-Threonine Kinases Proteins Sequence Homology, Amino Acid Wiskott-Aldrich Syndrome Protein cdc42 GTP-Binding Protein p21-Activated Kinases rac GTP-Binding Proteins metabolism metabolism metabolism |
|---|
| Mesh Heading Relevant : | metabolism metabolism metabolism methods isolation & purification isolation & purification |
|---|
Understanding the molecular basis of Wiskott-Aldrich syndrome.
(1998)
Journal - Cellular and molecular life sciences : CMLS (SWITZERLAND )
Abstract :
Wiskott-Aldrich syndrome (WAS) is an X-linked immunodeficiency disorder associated with lymphocytes and platelet abnormalities. The gene that encodes the Wiskott-Aldrich protein (WASP) was recently isolated, and shown to be defective in WAS patients. WASP contains multiple domains that interact with various signalling proteins, including the guanine triphosphatase (GTPase) Cdc42Hs and SH3 domain-containing proteins. Biochemical and genetic evidence strongly suggests that WASP is an important protein in the regulation of cell morphology. Recent progress in the identification of molecular partners for WASP suggests a molecular mechanism for the cellular abnormalities of WAS.
| ISSN : | 1420-682X |
|---|
| Mesh Heading : | Humans Lymphocyte Activation Proteins T-Lymphocytes Wiskott-Aldrich Syndrome Wiskott-Aldrich Syndrome Protein immunology immunology pathology |
|---|
| Mesh Heading Relevant : | Signal Transduction genetics genetics |
|---|
Reconstitution of cell-free NADPH oxidase activity by purified components.
(1995)
Journal - Methods in enzymology (UNITED STATES )
| ISSN : | 0076-6879 |
|---|
| Mesh Heading : | Cell Fractionation Cell Membrane Cell-Free System Cytochrome b Group Cytosol GTP-Binding Proteins Humans Macromolecular Substances Membrane Lipids Models, Biological NADH, NADPH Oxidoreductases NADPH Oxidase Neutrophils Phospholipids Recombinant Proteins Superoxides rac GTP-Binding Proteins methods enzymology ultrastructure blood isolation & purification enzymology ultrastructure biosynthesis isolation & purification blood chemistry isolation & purification ultrastructure blood biosynthesis isolation & purification metabolism blood |
|---|
| Mesh Heading Relevant : | metabolism blood enzymology |
|---|
Purification of Rac-GDP dissociation inhibitor complex from phagocyte cytosol.
(1995)
Journal - Methods in enzymology (UNITED STATES )
| ISSN : | 0076-6879 |
|---|
| Mesh Heading : | Animals Cell Fractionation Chromatography, Gel Chromatography, Ion Exchange Cytosol Electrophoresis, Polyacrylamide Gel GTP Phosphohydrolases GTP-Binding Proteins GTPase-Activating Proteins Guinea Pigs HL-60 Cells Humans Indicators and Reagents Macromolecular Substances Macrophages, Peritoneal Molecular Weight Neutrophils Phagocytes Proteins Ultracentrifugation rac GTP-Binding Proteins methods methods methods metabolism methods blood metabolism analysis metabolism analysis metabolism methods |
|---|
| Mesh Heading Relevant : | Guanine Nucleotide Dissociation Inhibitors isolation & purification isolation & purification metabolism metabolism metabolism isolation & purification |
|---|