Comparing the ability of a series of viral protein-expressing plasmid DNAs to protect against H5N1 influenza virus.
Journal - Virus genes (United States )
Avian influenza has been regarded as a human health threat. A major measure to prevent its outbreak is vaccination. In this study, a series of expression plasmids carrying the hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), matrix protein 1 (M1), and matrix protein 2 (M2) genes, respectively, of the avian influenza virus (AIV) A/Chicken/Henan/12/2004(H5N1) strain were constructed. These plasmids were administered to mice by electroporation (50 mug for each per administration, 1-5 times, at an interval of 2 weeks), and the mice were challenged with the homologous virus later. The mice immunized with HA plasmid once and the NA plasmid twice survived 100%, and those with NP plasmid showed 60-80% survival rate with at least three immunizations. The mice immunized with M1 plasmid survived 25% with five immunizations, while M2 plasmid had no protection even with five immunizations. The mixture of M1 and NP plasmids protected 95% of the mice against the homologous virus, and 80% of the mice against a challenge with heterologous H1N1 (PR8) virus. Moreover, the homologous protection lasted at least 6 months. Our data provided a basis for selecting multiple-component AIV vaccines.
|ISSN : ||0920-8569|
|Mesh Heading : ||Animals Antibodies, Viral Antigens, Viral Electroporation Female Hemagglutination Inhibition Tests Immunization, Secondary Influenza A Virus, H1N1 Subtype Influenza A Virus, H5N1 Subtype Influenza Vaccines Interferon-gamma Mice Mice, Inbred BALB C Survival Analysis T-Lymphocytes Time Factors Vaccines, DNA blood genetics immunology immunology genetics administration & dosage genetics secretion immunology administration & dosage genetics|
|Mesh Heading Relevant : ||immunology immunology immunology|