Physical interaction of human papillomavirus virus-like particles with immune cells.
Journal - International immunology (England )
Human papillomavirus virus-like particles (HPV VLP) and chimeric VLP are immunogens that are able to elicit potent anti-viral/tumor B and T cell responses. To investigate the immunogenicity of VLP, we determined which cells of the immune system are able to bind HPV-16 VLP. VLP were found to bind very well to human and mouse immune cells that expressed markers of antigen-presenting cells (APC) such as MHC class II, CD80 and CD86, including dendritic cells, macrophages and B cells. mAb blocking studies identified Fc gamma RIII (CD16) as one of the molecules to which the VLP can bind both on immune cells and foreskin epithelium. However, transfection of a CD16(-) cell line with CD16 did not confer binding of VLP. Splenocytes from Fc gamma RIII knockout mice showed a 33% decrease in VLP binding overall and specifically to subsets of APC. These combined data support a role for CD16 as an accessory molecule in an HPV VLP-receptor complex, possibly contributing to the immunogenicity of HPV VLP.
|ISSN : ||0953-8178|
|Mesh Heading : ||Animals Antibodies, Blocking Antigen-Presenting Cells B-Lymphocytes Base Sequence Cell Line Chimera DNA Primers Humans Male Mice Mice, Knockout Papillomaviridae Papillomavirus Infections Receptors, IgG Transfection Tumor Virus Infections immunology immunology immunology genetics pathogenicity immunology genetics metabolism immunology|
|Mesh Heading Relevant : ||immunology|