Membrane damaging potential of photosensitized riboflavin.
Journal - Indian journal of experimental biology (INDIA )
Riboflavin upon exposure to UV and visible radiations has been shown to produce active oxygen species. The present work deals with erythrocyte membrane as model system to study the damaging potential of photosensitized riboflavin. Membrane preparations (2.5 mg protein/ml) following exposure to sunlight in presence of riboflavin for different time intervals revealed significant inhibition of ATPases, p-nitrophenyl phosphatase and acetylcholinesterase. Considerable increase in lipid peroxidation was caused by the photosensitized riboflavin. Quenching studies using specific scavengers indicated remarkable inhibition. The production and identification of reactive oxygen species by photosensitized riboflavin and their possible involvement in membrane damaging effect has been discussed.
|ISSN : ||0019-5189|
|Mesh Heading : ||Erythrocyte Membrane Free Radicals Humans Light Oxygen Photochemistry Riboflavin enzymology metabolism radiation effects|
|Mesh Heading Relevant : ||drug effects pharmacology|
Isolation and characterization of basement membrane from frog skeletal muscle.
Journal - Preparative biochemistry (UNITED STATES )
Isolation of basement membrane from frog skeletal muscle has been described. The membrane preparation contained 35 micrograms hexoses, 1.72 micrograms sialic acid, 6.8 micrograms phospholipids, 0.21 micrograms cholesterol/mg protein. Na + K-ATPase and 5'-nucleotidase could not be detected in the membrane preparation. Glycine accounted for about 20% of the total amino acids. On SDS-PAGE, the membrane resolved into 20-22 polypeptide bands.
|ISSN : ||0032-7484|
|Mesh Heading : ||Amino Acids Animals Basement Membrane Carbohydrates Cholesterol Hydroxyproline Molecular Weight Muscles Phospholipids Proteins Ranidae Sarcolemma analysis analysis analysis analysis analysis analysis|
|Mesh Heading Relevant : ||analysis analysis analysis|