Effect of antibiotics on growth and laccase production from Cyathus bulleri and Pycnoporus cinnabarinus.
(2005)
Journal - Bioresource technology (England )
Abstract :
The effect of nine different antibiotics (chloramphenicol, ampicillin trihydrate, kanamycin A monosulfate, neomycin sulfate, erythromycin, thiostrepton, tetracycline, apramycin sulfate and streptomycin sulfate) on growth and laccase production from Cyathus bulleri and Pycnoporus cinnabarinus has been investigated. All the antibiotics tested at a concentration of 200 mg/l affected the fungal growth, release of protein and laccase production to different extent. Inhibition in fungal growth was found to be positively correlated with increase in laccase production. Interestingly, apramycin sulfate inhibited biomass production (14.9-26.2%), nevertheless, it stimulated maximum laccase production (18.2 U/ml) in both the fungi. Increasing concentrations of apramycin sulfate enhanced laccase production from P. cinnabarinus but not from C. bulleri.
| ISSN : | 0960-8524 |
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| Mesh Heading : | Anti-Bacterial Agents Basidiomycota Laccase Time Factors growth & development |
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| Mesh Heading Relevant : | pharmacology drug effects enzymology biosynthesis |
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Effect of amino acids and vitamins on laccase production by the bird's nest fungus Cyathus bulleri.
(2002)
Journal - Bioresource technology (England )
Abstract :
Various amino acids, their analogues and vitamins have shown stimulatory as well as inhibitory effects on laccase production by Cyathus bulleri. DL-methionine, DL-tryptophan, glycine and DL-valine stimulated laccase production, while L-cysteine monohydrochloride completely inhibited the enzyme production. Among vitamins tested biotin, riboflavin and pyridoxine hydrochloride were found to induce laccase production.
| ISSN : | 0960-8524 |
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| Mesh Heading : | Amino Acids Basidiomycota Fungal Proteins Laccase Oxidoreductases Sensitivity and Specificity Vitamins metabolism pharmacology biosynthesis metabolism pharmacology |
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| Mesh Heading Relevant : | drug effects enzymology biosynthesis |
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Ethidium bromide stimulated hyper laccase production from bird's nest fungus Cyathus bulleri.
(2002)
Journal - Letters in applied microbiology (England )
Abstract :
AIMS: Effect of ethidium bromide, a DNA intercalating agent, on laccase production from Cyathus bulleri was studied. METHODS AND RESULTS: The bird's nest fungus, Cyathus bulleri was grown on 2% (w/v) malt extract agar (MEA) supplemented with 1.5 microg ml(-1) of the phenanthridine dye ethidium bromide (EtBr) for 7 d and when grown subsequently in malt extract broth (MEB), produced a 4.2-fold increase in laccase production as compared to the untreated fungus. The fungal cultures following a single EtBr treatment, when regrown on MEA devoid of EtBr, produced a sixfold increase in laccase in MEB. However, on subsequent culturing on MEA in the absence of EtBr, only a 2.5-fold increase in laccase production could be maintained. In another attempt, the initial EtBr-treated cultures, when subjected to a second EtBr treatment (1.5 microg ml(-1)) on MEA for 7 d, produced a 1.4-fold increase in laccase production in MEB. CONCLUSIONS: The white-rot fungus Cyathus bulleri, when treated with EtBr at a concentration of 1.5 microg ml(-1) and regrown on MEA devoid of EtBr, produced a sixfold increase in laccase production in MEB. SIGNIFICANCE AND THE IMPACT OF THE STUDY: The variable form of C. bulleri capable of hyper laccase production can improve the economic feasibility of environmentally benign processes involving use of fungal laccases in cosmetics (including hair dyes), food and beverages, clinical diagnostics, pulp and paper industry, industrial effluent treatment, animal biotechnology and biotransformations.
| ISSN : | 0266-8254 |
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| Mesh Heading : | Animals Basidiomycota Ethidium Hot Temperature Hydrogen-Ion Concentration Laccase Models, Biological Mycoses Oxidoreductases drug effects growth & development etiology |
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| Mesh Heading Relevant : | enzymology pharmacology biosynthesis |
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