The spatial organization of centromeric heterochromatin during normal human lymphopoiesis: evidence for ontogenically determined spatial patterns.
Journal - Experimental cell research (United States )
It is believed that pericentromeric heterochromatin may play a major role in the epigenetic regulation of gene expression. We have previously shown that centromeres in human peripheral blood cells aggregate into distinct "myeloid" and "lymphoid" spatial patterns, suggesting that the three-dimensional organization of centromeric heterochromatin in interphase may be ontogenically determined during hematopoietic differentiation. To investigate this possibility, the spatial patterns of association of different centromeres were analyzed in hematopoietic progenitors and compared with those in early-B and early-T cells, mature B and T lymphocytes, and, additionally, mature granulocytes and monocytes. We show that those patterns change during lymphoid differentiation, with major spatial arrangements taking place at different stages during T and B cell differentiation. Heritable patterns of centromere association are observed, which can occur either at the level of the common lymphoid progenitor, or in early-T or early-B committed cells. A correlation of the observed patterns of centromere association with the gene content of the respective chromosomes further suggests that the variation in the composition of these heterochromatic structures may contribute to the dynamic relocation of genes in different nuclear compartments during cell differentiation, which might have functional implications for cell-stage-specific gene expression.
|ISSN : ||0014-4827|
|Mesh Heading : ||Antigens, CD34 B-Lymphocytes Cell Differentiation Cell Nucleus Centromere Chromosomes, Human Gene Expression Genes Granulocytes Hematopoietic Stem Cells Heterochromatin Humans Interphase Lymphopoiesis Monocytes Organ Specificity Stem Cells T-Lymphocytes metabolism cytology metabolism|
|Mesh Heading Relevant : ||cytology physiology physiology physiology cytology|