Determination of total iodine in biological material by alkaline ashing and column-switching ion-pair liquid chromatography.
Journal - Journal of chromatography. B, Biomedical sciences and applications (NETHERLANDS )
A method for the determination of total iodine in biological material has been developed. The method combines alkaline ashing with a selective and sensitive column-switching ion-pair HPLC technique. The ashing procedure which converts the organically bound iodine to iodide is miniaturised and requires only about 100 mg of sample. The first column of the column-switching system is polymer based and can therefore withstand the alkaline pH obtained after ashing. On the analytical column the iodide is separated as an ion-pair with tetrabutyl ammonium hydrogensulphate. The method has been applied to samples from whole blood, urine, liver, lung, carcass, and a sample throughput of at least 50 samples per day can be achieved. Validation studies by spiking experiments showed the precision to be better than 10% R.S.D. for all matrices with recoveries in the range 87-97%. The method has been applied for samples with an iodine content in the range 0.07-1060 microg/g.
|ISSN : ||1387-2273|
|Mesh Heading : ||Animals Bone and Bones Chromatography, High Pressure Liquid Hydroxides Iodine Liver Lung Potassium Compounds Rats Reproducibility of Results Sensitivity and Specificity Sodium Hydroxide Spectrophotometry, Ultraviolet Tissue Distribution chemistry methods blood urine chemistry chemistry|
|Mesh Heading Relevant : ||analysis|