T-cell receptor V beta expression is restricted in dermatitis herpetiformis skin.
(1997)
Journal - Acta dermato-venereologica (NORWAY )
Abstract :
An infiltrate of T-cells is found in lesional dermatitis herpetiformis skin, but the role of these cells in the pathogenesis of the skin lesions is unknown. The purpose of this study was to examine T-cell receptor V beta expression in skin biopsies taken from patients with dermatitis herpetiformis. Expression of eleven T-cell receptor V beta families in biopsies obtained from 10 patients was examined by immunoperoxidase staining and compared simultaneously with peripheral blood lymphocytes. Over-representation of V beta 2 (p < 0.02), V beta 5.2/5.3 (p < 0.01) and V beta 5.3 (p < 0.05) was found in lesional dermatitis herpetiformis skin compared with peripheral blood lymphocytes. These results suggest that recognition of an antigen(s) or superantigen is involved in the pathogenesis of dermatitis herpetiformis skin lesions.
| ISSN : | 0001-5555 |
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| Mesh Heading : | Adult Aged Dermatitis Herpetiformis Humans Middle Aged Receptors, Antigen, T-Cell, alpha-beta Skin T-Lymphocytes genetics immunology immunology |
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| Mesh Heading Relevant : | immunology analysis immunology |
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Failure of intradermal skin testing with gluten to produce delayed hypersensitivity reactions in patients with dermatitis herpetiformis.
(1995)
Journal - The British journal of dermatology (ENGLAND )
Abstract :
Dermatitis herpetiformis (DH) is characterized by a rash and a gluten-sensitive enteropathy (GSE) indistinguishable from that of coeliac disease. T-cell-mediated mechanisms have been implicated in the pathogenesis of GSE. It seems feasible that intradermal injection of gluten, in patients known to have GSE, could lead to an influx of T cells sensitized to gluten, with subsequent development of a delayed hypersensitivity-type reaction. Six patients with DH and three normal subjects had intradermal injections of 'Frazer's fraction III' (FFIII; the partial peptic tryptic digest of gluten which is known to be antigenic) and phosphate-buffered saline (PBS) as a control. Skin biopsies were taken at PBS and FFIII injection sites at 48 h. In addition, two of the patients with DH had biopsies taken of FFIII injection sites at 6 h. Monoclonal antibodies and the avidin-biotin-peroxidase technique were used to stain for T cells in the skin biopsies. A monoclonal antibody to a neoepitope exposed in the terminal complement complex and an immunofluorescent method were used to detect the presence of terminal complement component in biopsies taken from two of the control subjects and two of the patients. Both patients and control subjects developed a weal and flare within a few minutes of injecting the FFIII, and this persisted for up to 6 h. No skin reaction was present in either the patients or the control subjects at 48 h. No skin reaction was visible at any time following injection of PBS.(ABSTRACT TRUNCATED AT 250 WORDS)
| ISSN : | 0007-0963 |
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| Mesh Heading : | Antibodies Complement System Proteins Dermatitis Herpetiformis Female Gliadin Glutens Humans Hypersensitivity, Delayed Lymphocyte Count Male T-Lymphocytes analysis analysis immunology |
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| Mesh Heading Relevant : | Skin Tests immunology diagnostic use immunology |
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T lymphocytes in lesional skin of patients with dermatitis herpetiformis.
(1995)
Journal - The British journal of dermatology (ENGLAND )
Abstract :
Ten patients with dermatitis herpetiformis had biopsies taken from involved and uninvolved skin. Monoclonal antibodies and the avidin-biotin peroxidase staining technique were used to stain for T cells and Langerhans cells in skin sections. A significant increase in the number of CD3-positive T cells was observed in the upper dermis of involved compared with uninvolved skin (P < 0.0005). Most of the T cells in involved skin were CD45RO-positive memory cells; CD4-positive T cells exceeded the number of CD8-positive T cells by a ratio of 4:1. In addition, CD1a-positive dendritic cells were observed within the clumps of T cells in involved dermis in nine of the 10 patients, but were absent from the dermis of uninvolved skin. Double immunofluorescent staining demonstrated that approximately 20-40% of the CD3-positive T cells were activated, and expressed the HLA-DR antigen. These findings suggest that activated T cells are involved in the pathogenesis of dermatitis herpetiformis skin lesions.
| ISSN : | 0007-0963 |
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| Mesh Heading : | Antigens, CD3 Antigens, CD45 Dermatitis Herpetiformis Fluorescent Antibody Technique HLA-DR Antigens Humans Immunoenzyme Techniques Lymphocyte Activation Lymphocyte Count Skin T-Lymphocytes analysis analysis pathology analysis pathology pathology |
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| Mesh Heading Relevant : | immunology immunology immunology |
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25 years' experience of a gluten-free diet in the treatment of dermatitis herpetiformis.
(1994)
Journal - The British journal of dermatology (ENGLAND )
Abstract :
Gluten-free diets have been used in the treatment of patients with dermatitis herpetiformis in our department since 1967. Of the 212 patients with dermatitis herpetiformis attending between 1967 and 1992, 133 managed to take the diet, and 78 of these achieved complete control of their rash by diet alone. Of the remaining 55 patients taking a gluten-free diet, all but three were taking partial diets; over half of these patients managed to substantially reduce the dose of medication required. Of the 77 patients taking a normal diet, eight entered spontaneous remission, giving a remission rate of 10%; a further two patients who had been taking gluten-free diets were found to have remitted when they resumed normal diets. Loss of IgA from the skin was observed in 10 of 41 (24%) patients taking strict gluten-free diets. These patients had been taking their diets for an average of 13 years (range 5-24 years), and their rash had been controlled by diet alone for an average of 10 years (range 3-16 years). The advantages of a gluten-free diet in the management of patients with dermatitis herpetiformis are: (i) the need for medication is reduced or abolished; (ii) there is resolution of the enteropathy, and (iii) patients experience a feeling of well-being after commencing the diet. Thus, we propose that a gluten-free diet is the most appropriate treatment for patients with dermatitis herpetiformis.
| ISSN : | 0007-0963 |
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| Mesh Heading : | Adolescent Adult Aged Aged, 80 and over Celiac Disease Child Dermatitis Herpetiformis Diet Female Follow-Up Studies Humans Immunoglobulin A Intestine, Small Male Middle Aged Skin Time Factors complications diet therapy immunology pathology complications immunology pathology metabolism pathology immunology |
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| Mesh Heading Relevant : | Glutens diet therapy |
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