Molecular fingerprinting of Vibrio tapetis strains using three PCR-based methods: ERIC-PCR, REP-PCR and RAPD.
Journal - Diseases of aquatic organisms (Germany )
Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clams and causes heavy economic losses. In this study, 28 V. tapetis strains isolated from 5 different hosts were intraspecifically characterized by 3 different polymerase chain reaction- (PCR-) based typing methods: enterobacteria repetitive intergenic consensus (ERIC)-PCR, repetitive extragenic palindromic (REP)-PCR and randomly amplified polymorphic DNA (RAPD)-PCR. Cluster analysis of genetic profiles obtained from these molecular techniques clearly showed the existence of 3 genetic groups strongly correlated to the host origin. The first group was formed by 23 V. tapetis strains isolated from Manila clam Ruditapes philippinarum, 1 isolated from venus clam Venerupis aurea, and 1 isolated from common cockle Cerastoderma edule, all collected from France and Spain. The second group was formed by 2 strains isolated from carpet-shell clam R. decussatus cultured in the northwest of Spain. The third group was composed of 1 strain isolated from Atlantic halibut Hippoglossus hippoglossus from the UK. We concluded that the 3 typing methods based on PCR were useful for the intraspecific typing of V. tapetis strains, and that they can potentially be used as a fast and reliable tool for epidemiological studies in the future.
|ISSN : ||0177-5103|
|Mesh Heading : ||Animals Bivalvia DNA Fingerprinting DNA Primers DNA, Bacterial Electrophoresis, Agar Gel Epidemiology, Molecular Flounder Genetic Variation Polymerase Chain Reaction Random Amplified Polymorphic DNA Technique Vibrio microbiology methods chemistry genetics veterinary microbiology genetics veterinary|
|Mesh Heading Relevant : ||veterinary methods veterinary classification genetics|
Cognitive functioning, family history of alcoholism, and antisocial behavior in female polydrug abusers.
Journal - Psychological reports (UNITED STATES )
Family history of alcoholism and antisocial behaviour had both independent and synergic negative relationships with abstract verbal reasoning for a group of 42 female polydrug abusers whose main drug was heroin. This finding suggests that the observed lower cognitive performance may reflect the family history of alcoholism or antisocial behaviour rather than just drug abuse and is consistent with theories predicting a relative deficiency in high language skill by persons with antisocial personality disorders.
|ISSN : ||0033-2941|
|Mesh Heading : ||Adult Alcoholism Antisocial Personality Disorder Child of Impaired Parents Comorbidity Female Heroin Dependence Humans Risk Factors Substance-Related Disorders genetics rehabilitation genetics rehabilitation genetics rehabilitation adverse effects adverse effects diagnosis genetics rehabilitation|
|Mesh Heading Relevant : ||Neuropsychological Tests Psychotropic Drugs Street Drugs psychology psychology psychology psychology psychology|