Expression of multiple c-kit receptor messenger ribonucleic acid transcripts during postnatal development of the rat testis.
Journal - Biochemical and biophysical research communications (UNITED STATES )
The c-kit protooncogene is a transmembrane tyrosine kinase receptor expressed during gametogenesis. Using the polymerase chain reaction (PCR), we have identified the c-kit receptor mRNA transcripts in the rat testis and studied their expression during postnatal development of the testis. Five different transcripts were identified using sets of primers encoding within the extracellular domain. Two transcripts were obtained from primer sets encoding regions within the cytoplasmic domain and the primer set encoding the entire length of the c-kit receptor. We have compared the levels of expression of these transcripts on different days during postnatal development. The level of expression of a particular transcript varied depending upon the developmental stage of the testis. In summary, our results suggest that multiple forms of mRNAs exist for the c-kit receptor in the rat testis, and they are regulated differentially during postnatal development.
|ISSN : ||0006-291X|
|Mesh Heading : ||Aging Animals Base Sequence Cell Membrane DNA Primers Male Molecular Sequence Data Molecular Weight Polymerase Chain Reaction Proto-Oncogene Proteins c-kit RNA, Messenger Rats Testis Transcription, Genetic metabolism isolation & purification metabolism biosynthesis growth & development|
|Mesh Heading Relevant : ||Gene Expression metabolism biosynthesis metabolism|