The putative cancer stem cell marker USP22 is a subunit of the human SAGA complex required for activated transcription and cell-cycle progression.
Journal - Molecular cell (United States )
Polycomb genes encode critical regulators of both normal stem cells and cancer stem cells. A gene signature that includes Polycomb genes and additional genes coregulated with Polycomb genes was recently identified. The expression of this signature has been reported to identify tumors with the cancer stem cell phenotypes of aggressive growth, metastasis, and therapy resistance. Most members of this 11 gene signature encode proteins with well-defined roles in human cancer. However, the function of the signature member USP22 remains unknown. We report that USP22 is a previously uncharacterized subunit of the human SAGA transcriptional cofactor complex. Within SAGA, USP22 deubiquitylates histone H2B. Furthermore, USP22 is recruited to specific genes by activators such as the Myc oncoprotein, where it is required for transcription. In support of a functional role within the Polycomb/cancer stem cell signature, USP22 is required for appropriate progression through the cell cycle.
|ISSN : ||1097-2765|
|Mesh Heading : ||Adaptor Proteins, Signal Transducing Biological Markers Carcinoma, Non-Small-Cell Lung Cell Cycle Cell Line, Transformed Cell Line, Tumor Histone Acetyltransferases Humans Lung Neoplasms Neoplastic Stem Cells Nuclear Proteins Promoter Regions, Genetic Protein Processing, Post-Translational Protein Subunits Proto-Oncogene Proteins c-myc Recombinant Fusion Proteins Thiolester Hydrolases Trans-Activators Transcription Factors Transcription, Genetic Ubiquitination p300-CBP Transcription Factors analysis physiology pathology cytology metabolism cytology metabolism analysis physiology pathology cytology analysis physiology genetics physiology physiology physiology analysis physiology|
|Mesh Heading Relevant : ||Gene Expression Profiling physiology metabolism physiology physiology physiology chemistry physiology physiology|