Use of interrepeat PCR fingerprinting to investigate an Acinetobacter baumannii outbreak in an intensive care unit.
Journal - Scandinavian journal of infectious diseases (SWEDEN )
An epidemiological investigation of an outbreak of Acinetobacter baumannii among patients on 2 closely related intensive care units (ICU) was performed by molecular typing with interrepeat polymerase chain reaction (interrepeat PCR). 31 A. baumannii isolates obtained from 15 ICU patients were characterized. All patients were infected or colonized with A. baumannii. After identification of the outbreak, 6 environmental isolates were collected from tap-water, sinks and cleaning detergents. PCR fingerprinting identified 3 genotypes among the outbreak-related strains. One predominant genotype was demonstrated in 14/15 patients and this genotype was also found among all environmental isolates. The cluster of A. baumannii represented an outbreak of 1 genotype, suggesting cross-contamination. The finding of the identical genotype among all environmental strains indicated a common environmental source causing the outbreak. The outbreak was controlled after reimplementation of an effective disinfection of workplace surfaces. This survey proved interrepeat PCR to be a rapid and reliable method to differentiate A. baumannii strains, thereby allowing epidemiological surveillance of large amounts of strains and early interventions to control outbreaks.
|ISSN : ||0036-5548|
|Mesh Heading : ||Acinetobacter Acinetobacter Infections Cross Infection DNA Fingerprinting Genotype Hospitals, University Humans Incidence Intensive Care Units Netherlands Polymerase Chain Reaction genetics isolation & purification diagnosis diagnosis methods|
|Mesh Heading Relevant : ||Disease Outbreaks classification epidemiology epidemiology methods|
Epidemiological survey of an outbreak of multiresistant Serratia marcescens by PCR-fingerprinting.
Journal - Infection (GERMANY )
During an outbreak of Serratia marcescens from May to November 1993 43 strains obtained from 27 ICU patients infected or colonized with multiresistant S. marcescens were genotypically characterized with random amplified polymerase chain reaction (RAPD-PCR)-fingerprinting. In addition, 43 epidemiologically unrelated control isolates were selected. PCR-fingerprinting identified ten different genotypes of S. marcescens among the outbreak related strains. One predominant genotype was demonstrated in 21/43 isolates of 11/27 patients. A cluster of this genotype was found in seven/eight patients on the cardiosurgical ICU. The epidemiologically unrelated strains all showed different genotypes as compared to the predominant type. This survey proved RAPD-PCR to be a highly discriminatory and reproducible method for epidemiological studies of S. marcescens strains in nosocomial outbreaks.
|ISSN : ||0300-8126|
|Mesh Heading : ||Bacterial Typing Techniques Base Sequence DNA Primers DNA, Bacterial Drug Resistance, Multiple Genotype Humans Intensive Care Units Molecular Sequence Data Reproducibility of Results Serratia Infections beta-Lactam Resistance methods microbiology drug effects|
|Mesh Heading Relevant : ||DNA Fingerprinting Disease Outbreaks Random Amplified Polymorphic DNA Technique Serratia marcescens epidemiology|