A comparative study on IgG-ELISA, IgM-IFT and Kato-Katz methods for epidemiological purposes in a low endemic area for schistosomiasis.
Journal - Memórias do Instituto Oswaldo Cruz (BRAZIL )
The high sensitivity and the possibility of automation of the enzyme-linked-immunosorbent-assay (ELISA) has indicated this technique as one of the most useful serological test for epidemiological studies. In the present study, an ELISA for detection of IgG antibodies against adult worm antigens (IgG-ELISA) was investigated for epidemiological purposes, in a rural area of the municipality of Itariri (São Paulo, Brazil). Blood on filter paper (1,180 samples) from about 650 schoolchildren were submitted to ELISA and the data compared to the results of the parasitological method of Kato-Katz and also to the IgM-IFT (immunofluorescence test for IgM antibodies to gut associated antigens). The prevalence rates respectively of 8.5%, 43.0% and 56.2% by the Kato-Katz, IgG-ELISA, and IgM-IFT methods suggest the poor sensitivity of the parasitological method for detection of Schistosoma mansoni eggs in individuals with low worm burden, situation commonly observed in low endemic areas. These results can partially explain the poor degree of agreement between the IgG-ELISA and the Kato-Katz, as suggested by the Kappa index of 0.170. Otherwise, the Kappa index of 0.675 showed substantial agreement between the two serological tests. Some discrepancy of results between the two serological techniques must be better investigated.
|ISSN : ||0074-0276|
|Mesh Heading : ||Animals Antibodies, Helminth Brazil Child Endemic Diseases Feces Humans Immunoglobulin G Immunoglobulin M Rural Population Schistosoma mansoni Schistosomiasis mansoni Sensitivity and Specificity Seroepidemiologic Studies parasitology epidemiology parasitology|
|Mesh Heading Relevant : ||Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique blood blood blood immunology diagnosis|