Endometrial dating and determination of the window of implantation in healthy fertile women.
Journal - Fertility and sterility (UNITED STATES )
OBJECTIVE: To reassess endometrial morphological criteria of normality identifying the best morphological and molecular "implantation window" indicators in normal women. DESIGN: Prospective clinical study.Setting: Assisted reproductive unit. PATIENT(s): Fourteen healthy volunteers. INTERVENTION(s): Blood sampling for LH, E(2), and progesterone (P4) determinations. Daily vaginal ultrasounds. Two endometrial biopsies per volunteer, 7 days apart, during luteal phase. MAIN OUTCOME MEASURE(s): Endometrial dating, pinopodes formation, immunohistochemical determination of integrins (alphavbeta3, alpha4beta1), leukemia inhibitory factor (LIF), interleukin-1 receptor type I (IL-1R tI), mouse ascites Golgi (MAG), the transmembrane mucin (MUC-1), and P4 receptor expression. RESULT(s): In 26 of 28 biopsies observers agreed; in two biopsies there was a discrepancy (difference of 72 hours). With use of LH peak, 24 of 26 samples were in phase, and 2 were 3 days behind. Pinopodes appeared on days 20-21 and persisted through day 28 in small groups or larger areas. beta3 Integrin was highly expressed in luminal and glandular epithelium from day 22 through 28; 48 hours thereafter pinopodes appeared. alpha4 Subunit exhibited luminal epithelium reaction positivity on days 22-23 and glands on days 18-23. LIF and IL-1R tI showed weak, erratic expression. MAG antibodies showed luminal epithelium expression up to day 22 and glands up to day 25. MUC-1 showed positivity during the whole luteal phase. P4 receptors were positive through day 20 and at the end of the luteal phase. CONCLUSION(s): The three most cited markers that frame the window of implantation do not correlate in our material. Pinopodes are present from day 20 on; beta3 and alpha4 integrin subunits indicate a window opening on days 22-23.
|ISSN : ||0015-0282|
|Mesh Heading : ||Adult Endometrium Estradiol Female Follicle Stimulating Hormone Golgi Apparatus Growth Inhibitors Humans Immunohistochemistry Integrin alpha4beta1 Integrins Leukemia Inhibitory Factor Luteinizing Hormone Lymphokines Menstrual Cycle Microscopy, Electron, Scanning Mucins Prospective Studies Receptors, Interleukin-1 Receptors, Interleukin-1 Type I Receptors, Lymphocyte Homing Receptors, Progesterone Receptors, Vitronectin Reference Values anatomy & histology blood blood immunology metabolism methods metabolism blood metabolism metabolism metabolism metabolism metabolism metabolism|
|Mesh Heading Relevant : ||Embryo Implantation Interleukin-6 physiology physiology|