Analysis of paralogous pontin and reptin gene expression during mouse development.
Journal - Development genes and evolution (Germany )
Evolutionarily conserved from yeast to human, the paralogous DNA helicases Pontin (Pont) and Reptin (Rept) are simultaneously recruited in multi-protein chromatin complexes that function in different aspects of DNA metabolism (transcription, replication and repair). When assayed, the two proteins were found to be essential for viability and to play antagonistic roles, suggesting that the balance of Pont/Rept regulates epigenetic programmes critical for development. Consistent with this, the two helicases are provided in the same embryonic territories during Drosophila development. In Xenopus, while transcribed in the same regions early in embryogenesis, pont and rept adopt significantly different patterns afterwards. Here we report that the two genes follow highly resembling transcription patterns in mouse embryos, with prominent expression in limb buds and branchial arches, organs undergoing mesenchymal-epithelial interactions and in motoneurones from cranial and spinal regions. Thus, simultaneous expression during development appears to constitute another feature of the evolutionary conservation of pont and rept genes.
dlarp, a new candidate Hox target in Drosophila whose orthologue in mouse is expressed at sites of epithelium/mesenchymal interactions.
Journal - Developmental dynamics : an official publication of the American Association of Anatomists (UNITED STATES )
Hox complex genes are key developmental regulators highly conserved throughout evolution. They encode transcription factors that initiate genetic programs of diversified morphogenesis along the anteroposterior embryonic axis. We report the characterization of the novel Drosophila Hox target gene dlarp, isolated from a further screen of a previously described library of genomic DNA fragments associated in vivo with Ultrabithorax proteins. The dlarp spatio-temporal pattern of transcription in wild-type and homeotic mutant embryos is consistent with a positive regulation by Sex combs reduced and Ultrabithorax in the parasegment 2 ectoderm and the abdominal mesoderm, respectively. The teashirt gene product, thought to act in concert with Hox proteins, is also required for the transcriptional control of this target. Search in databases revealed that dlarp has been highly conserved during evolution. The embryonic expression pattern of the mouse orthologue does not support a function downstream of Hox proteins. It is mainly transcribed in neural structures and in developing organs characterized by epithelial-mesenchymal interactions.
|ISSN : ||1058-8388|
|Mesh Heading : ||Amino Acid Sequence Animals Autoantigens Base Sequence Body Patterning Cloning, Molecular DNA DNA-Binding Proteins Drosophila Embryo, Nonmammalian Epithelium Evolution, Molecular Gene Expression Regulation, Developmental Genes, Insect Homeodomain Proteins Humans In Situ Hybridization Insect Proteins Mesoderm Mice Molecular Sequence Data Rats Recombinant Fusion Proteins Ribonucleoproteins Transcription Factors chemistry genetics metabolism analysis isolation & purification metabolism embryology metabolism ultrastructure anatomy & histology genetics physiology metabolism metabolism cytology chemistry genetics metabolism chemistry metabolism|
|Mesh Heading Relevant : ||Drosophila Proteins Repressor Proteins genetics genetics metabolism metabolism genetics|