A PHYSICAL APPROACH TO GENOMIC ANALYSIS
(1982)
Abstract :
The goal of this research is to perfect quantitative methods for the physical characterization of simple DNAs and to extend thermal denaturation methods by combining efficient approaches to the mapping of thermalites with additional information about the composition and sequence biases within thermalites to obtain a new type of description for genomic units, to be referred to as the "sequence constitution." It is theorized that the sequence constitution may emphasize features of the base pair sequence that are related to the control functions of the nucleic acid. We plan to use single strand reagents and polarographic studies to correlate the presence of unique tertiary structural features at premelting temperatures with deductions about sequence constitution arrived at by thermal denaturation analysis. In addition, working in collaboration with a theoretician, we hope to refine existing theory on the mechanism of helix coil transitions in DNA.
| Project Number : | 5R01GM023067-05 |
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| ICD : | NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES |
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| IRG : | BBCA |
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| Project Terms : | BIOPHYSICS AND BIOPHYSICAL CHEMISTRY A STUDY SECTION, GENETICS, CHROMOSOME COMPLEMENT, GENOME, NUCLEIC ACIDS STRUCTURE, NUCLEOSIDES (TIDES) SEQUENCE, TEMPERATURE, HEAT STABILITY-LABILITY BIOLOGY, SYSTEMATIC, EVOLUTION AND PHYLOGENY, GENETICS, GENES, NUCLEIC ACIDS, DNA BACTERIAL, NUCLEIC ACIDS, DNA VIRAL, NUCLEIC ACIDS, MT DNA BACTERIA (GENERAL), CELL COMPONENTS, CHLOROPLASTS, GENETICS, EXTRACHROMOSOMAL INHERITANCE, PLASMIDS, GENETICS, MUTATION, MUTANTS, MAMMALS, NUCLEIC ACIDS DENATURATION, VIRUSES, BACTERIOPHAGE, VIRUSES, ONCOGENIC (INCLUDING ONCOGENIC VIRUS-LIKE PARTICLES) |
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A PHYSICAL APPROACH TO GENOMIC ANALYSIS
(1982)
Abstract :
The goal of this research is to perfect quantitative methods for the physical characterization of simple DNAs and to extend thermal denaturation methods by combining efficient approaches to the mapping of thermalites with additional information about the composition and sequence biases within thermalites to obtain a new type of description for genomic units, to be referred to as the "sequence constitution." It is theorized that the sequence constitution may emphasize features of the base pair sequence that are related to the control functions of the nucleic acid. We plan to use single strand reagents and polarographic studies to correlate the presence of unique tertiary structural features at premelting temperatures with deductions about sequence constitution arrived at by thermal denaturation analysis. In addition, working in collaboration with a theoretician, we hope to refine existing theory on the mechanism of helix coil transitions in DNA.
| Project Number : | 5R01GM023067-06 |
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| ICD : | NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES |
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| IRG : | BBCA |
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| Project Terms : | GENETICS, CHROMOSOME COMPLEMENT, GENOME, MOLECULAR AND CELLULAR BIOPHYSICS STUDY SECTION, NUCLEIC ACIDS STRUCTURE, NUCLEOSIDES (TIDES) SEQUENCE, TEMPERATURE, HEAT STABILITY-LABILITY BIOLOGY, SYSTEMATIC, EVOLUTION AND PHYLOGENY, GENETICS, GENES, NUCLEIC ACIDS, DNA BACTERIAL, NUCLEIC ACIDS, DNA VIRAL, NUCLEIC ACIDS, MT DNA BACTERIA (GENERAL), CELL COMPONENTS, CHLOROPLASTS, GENETICS, EXTRACHROMOSOMAL INHERITANCE, PLASMIDS, GENETICS, MUTATION, MUTANTS, MAMMALS, NUCLEIC ACIDS DENATURATION, VIRUSES, BACTERIOPHAGE, VIRUSES, ONCOGENIC (INCLUDING ONCOGENIC VIRUS-LIKE PARTICLES) |
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A PHYSICAL APPROACH TO GENOMIC ANALYSIS
(1982)
Abstract :
The research proposed applies the most recent techniques for high resolution thermal denaturation of DNA to the physical characterization of simple genomes. In addition, the experiments should test the validity of the hypothesis that thermal subtransitions in DNA are related to individual genes and investigate the possibility that unique physical structures at local sites in DNA may determine the ends of a cooperatively interacting sequence of bases. This investigation has been proposed because recent advances in methods for recording and analyzing thermal transitions of pure DNA provide denaturation profiles that contain vastly more information than previously was found in traditional melting curves.
| Project Number : | 2R01GM023067-04 |
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| ICD : | NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES |
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| IRG : | BBCA |
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| Project Terms : | BIOPHYSICS AND BIOPHYSICAL CHEMISTRY STUDY SECTION, GENETICS, CHROMOSOME COMPLEMENT, GENOME, NUCLEIC ACIDS STRUCTURE, NUCLEOSIDES (TIDES) SEQUENCE, TEMPERATURE, HEAT STABILITY-LABILITY BIOLOGY, SYSTEMATIC, EVOLUTION AND PHYLOGENY, GENETICS, GENES, NUCLEIC ACIDS, DNA BACTERIAL, NUCLEIC ACIDS, DNA VIRAL, NUCLEIC ACIDS, MT DNA BACTERIA (GENERAL), CELL COMPONENTS, CHLOROPLASTS, GENETICS, EXTRACHROMOSOMAL INHERITANCE, EPISOMES, GENETICS, MUTATION, MUTANTS, MAMMALS, NUCLEIC ACIDS DENATURATION, VIRUSES, BACTERIOPHAGE, VIRUSES, ONCOGENIC (INCLUDING ONCOGENIC VIRUS-LIKE PARTICLES) |
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A PHYSICAL APPROACH TO GENOMIC ANALYSIS
(1979)
Abstract :
Recent advances in methods for recording and analyzing thermal transitions of pure DNA provide denaturation profiles that contain vastly more information than found in traditional melting curves. Quantitatively recorded profiles plotting the first derivative of DNA hyperchromicity vs. temperature show that the thermal transition of simple DNAs consists of a series of narrow, partially overlapping peaks, referred to as thermalites. In properly performed experiments, the area under each thermalite is closely proportional to the number of base pairs involved in an individual cooperative subtransition of the total denaturation process. In favorable cases, the number, position, breadth, and area of each thermalite can be measured to provide a highly detailed physical characterization that is determined by the sequences of the bases in the DNA. An analysis of current profiles suggests the hypothesis that each thermalite may actually represent an individual gene. This unexpected circumstance might arise if genes are "punctuated" at their ends by special sequences which give rise at elevated temperatures to unique physical structures with physical structures with increased thermal stability. The research proposed would apply the most recent techniques for high-resolution thermal denaturation of DMA to the physical characterization of simple genomes. In addition, the experiments would test the validity of the hypothesis that thermal subtransitions in DNA are related to individual genes and investigate the possiblitity that unique physical structures at local sites in DNA may determine the ends of cooperatively interacting sequence bases.
| Project Number : | 1R01GM023067-01 |
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| ICD : | NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES |
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| IRG : | BBCA |
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| Project Terms : | BIOPHYSICS AND BIOPHYSICAL CHEMISTRY STUDY SECTION, GENETICS, CHROMOSOME COMPLEMENT, GENOME, NUCLEIC ACIDS STRUCTURE, NUCLEOSIDES (TIDES) SEQUENCE, TEMPERATURE, HEAT STABILITY-LABILITY BIOLOGY, SYSTEMATIC, EVOLUTION AND PHYLOGENY, GENETICS, GENES, NUCLEIC ACIDS, DNA BACTERIAL, NUCLEIC ACIDS, DNA VIRAL, NUCLEIC ACIDS, MT DNA BACTERIA (GENERAL)*, CELL COMPONENTS, CHLOROPLASTS, GENETICS, EXTRACHROMOSOMAL INHERITANCE, EPISOMES, GENETICS, MUTATION, MUTANTS*, MAMMALS*, NUCLEIC ACIDS DENATURATION, VIRUSES, BACTERIOPHAGE*, VIRUSES, ONCOGENIC (INCLUDING ONCOGENIC VIRUS-LIKE PARTICLES) |
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A PHYSICAL APPROACH TO GENOMIC ANALYSIS
(1979)
Abstract :
The research proposed applies the most recent techniques for high resolution thermal denaturation of DNA to the physical characterization of simple genomes. In addition, the experiments should test the validity of the hypothesis that thermal subtransitions in DNA are related to individual genes and investigate the possibility that unique physical structures at local sites in DNA may determine the ends of cooperatively denaturing sequences of bases. The research should contribute to understanding genetic features of cancer viruses at a molecular level.
| Project Number : | 5R01GM023067-02 |
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| ICD : | NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES |
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| IRG : | BBCA |
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| Project Terms : | BIOPHYSICS AND BIOPHYSICAL CHEMISTRY STUDY SECTION, GENETICS, CHROMOSOME COMPLEMENT, GENOME, NUCLEIC ACIDS STRUCTURE, NUCLEOSIDES (TIDES) SEQUENCE, TEMPERATURE, HEAT STABILITY-LABILITY BIOLOGY, SYSTEMATIC, EVOLUTION AND PHYLOGENY, GENETICS, GENES, NUCLEIC ACIDS, DNA BACTERIAL, NUCLEIC ACIDS, DNA VIRAL, NUCLEIC ACIDS, MT DNA BACTERIA (GENERAL)*, CELL COMPONENTS, CHLOROPLASTS, GENETICS, EXTRACHROMOSOMAL INHERITANCE, EPISOMES, GENETICS, MUTATION, MUTANTS*, MAMMALS*, NUCLEIC ACIDS DENATURATION, VIRUSES, BACTERIOPHAGE*, VIRUSES, ONCOGENIC (INCLUDING ONCOGENIC VIRUS-LIKE PARTICLES) |
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A PHYSICAL APPROACH TO GENOMIC ANALYSIS
(1979)
Abstract :
The research proposed applies the most recent techniques for high resolution thermal denaturation of DNA to the physical characterization of simple genomes. In addition, the experiments should test the validity of the hypothesis that thermal subtransitions in DNA are related to individual genes and investigate the possibility that unique physical structures at local sites in DNA may determine the ends of a cooperatively interacting sequence of bases. This investigation has been proposed because recent advances in methods for recording and analyzing thermal transitions of pure DNA provide denaturation profiles that contain vastly more information than previously was found in traditional melting curves.
| Project Number : | 5R01GM023067-03 |
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| ICD : | NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES |
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| IRG : | BBCA |
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| Project Terms : | BIOPHYSICS AND BIOPHYSICAL CHEMISTRY STUDY SECTION, GENETICS, CHROMOSOME COMPLEMENT, GENOME, NUCLEIC ACIDS STRUCTURE, NUCLEOSIDES (TIDES) SEQUENCE, TEMPERATURE, HEAT STABILITY-LABILITY BIOLOGY, SYSTEMATIC, EVOLUTION AND PHYLOGENY, GENETICS, GENES, NUCLEIC ACIDS, DNA BACTERIAL, NUCLEIC ACIDS, DNA VIRAL, NUCLEIC ACIDS, MT DNA BACTERIA (GENERAL), CELL COMPONENTS, CHLOROPLASTS, GENETICS, EXTRACHROMOSOMAL INHERITANCE, EPISOMES, GENETICS, MUTATION, MUTANTS, MAMMALS, NUCLEIC ACIDS DENATURATION, VIRUSES, BACTERIOPHAGE, VIRUSES, ONCOGENIC (INCLUDING ONCOGENIC VIRUS-LIKE PARTICLES) |
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ANALYSIS OF ULTRACENTRIFUGATION DATA
(1972)
| Project Number : | 5R01CA011430-070022 |
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| ICD : | NATIONAL CANCER INSTITUTE |
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| Project Terms : | PHYSICAL SEPARATION, ULTRACENTRIFUGATION* COMPUTER ANALYSIS* |
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